علوم زیستی دریا
Leala Alami-Naysi; Mohammad Ali Salari-Aliabadi
Abstract
In order to investigate the population structure species of sea cucumber Holothuria parva in the two regions Bostaneh Port and Dayer Port, 16S rRNA gene sequencing method was used. In total, 417 nucleotide locus were determined, after investigating in the NCBI database, sequences were consistent with ...
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In order to investigate the population structure species of sea cucumber Holothuria parva in the two regions Bostaneh Port and Dayer Port, 16S rRNA gene sequencing method was used. In total, 417 nucleotide locus were determined, after investigating in the NCBI database, sequences were consistent with 16S rRNA gene and Samples were verified to belong to the species of H. parva. In total four haplotypes were identified, one of which was common in both regions. Bostaneh Port had 3 haplotypes and Dayer Port had 2 haplotypes. Haplotype diversity was estimated to be 83 percent in Bostaneh Port and 50 percent in Dayer Port. Estimated Nucleotide diversity in Bostaneh and Dayer Ports were measured 0.007 and 0.002 respectively. Low genetic differentiation (Fst=0.000), divergences rate (Dxy= 0.0048) and high gene flow (Nm=1874) between the two regions were estimated. Based on this study Bostaneh and Dayer Port samples have probably diverged from an identical population since a high gene flow and low differentiation is observed among them. There is also a common haplotype, suggesting a common ancestor of H. parva in two regions.
Iman Ghanavati Mohammad Ghasemi; HOSEIN ZOLGHARNEIN; hoda Khaledi
Abstract
In order to study the genetic diversity of Hara trees in the coastal waters of Hormozgan province, using 40 RAPD molecular samples, 40 samples of Hara leaves were collected at different stations (ports of Jask, Dumitry, Tiba and Qeshm Island) and transferred to the laboratory. After extraction of DNA ...
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In order to study the genetic diversity of Hara trees in the coastal waters of Hormozgan province, using 40 RAPD molecular samples, 40 samples of Hara leaves were collected at different stations (ports of Jask, Dumitry, Tiba and Qeshm Island) and transferred to the laboratory. After extraction of DNA They were examined by 30 primers in polymerase chain reaction. The results showed that the highest mean genetic variation in Qeshm population was 0 458 with a standard deviation of 0.033 and the lowest mean of genetic diversity in Jask population was 0.423 with a standard deviation of 0.056. Also, using the PopGene software, the average Shannon information index was 0.643 for four populations. The value of Fst index was 011/0 and the gene flow rate was 25.854, and the UPGMA dendrogram, based on the Nei genetic distance, did not show a clear separation between the populations. Also, molecular variance analysis showed that there is a high variation (99%) within the studied populations. The results of this research can provide useful information for conservation and management programs of this kind with indigenous value of Iran